Oroboros O2k-Fluo Series J and Power-Oroboros O2k-Fluo Series J

Procurement Summary

The National Institute on Aging (NIA) requires Clinical Research unit medical staff to perform muscle biopsies on participants in the GESTALT, BLSA, Tissue Procurement and the Protein Turnover studies. The instruments with multi parameter analysis and highest oxygen concentration resolution produce a detailed snapshot of mitochondrial bioenergetics in muscle biopsy studies that can be used as a fingerprint of mitochondrial activity. The salient characteristics are as follows: Integrated computer with touchscreen connects DatLab to the O2k Keyboard, webcam and webcam accessories Item 1: O2k: Hardware and DatLab Software • Stainless steel housing • Peltier thermostat 4 to 47 °C; ±0.002 °C • Barometric pressure transducer • Amperometric oxygen channels • ISE sockets for ion sensitive electrodes • Sockets for optical signals • Sockets for PB-Module • Duran glass chambers (2 mL) • SmartPOS: Polarographic Oxygen Sensor (POS) with Connector • PVDF stirrers, electromagnetic control • PEEK stoppers with O-ring • Blue POM chamber holders with O-ring and V-ring • Blue POM OroboPOS-holders • Current DatLab version with free follow-up upgrades within the warranty period O2k: Accessories and Consumables • ISS-Integrated Suction System, to remove medium from the O2k-Chamber • Oxia - the O2 regime controller for HyperOxia and HypOxia • O2k-Titration Set: Hamilton microsyringes; fixed needles with rounded tips • MiR05-Kit: Mitochondrial Respiration Medium O2k-Fluo Smart-Module: an amperometric add-on module for measurement of hydrogen peroxide production, ATP production, mt-membrane potential, Ca2+ • Smart Fluo-Sensors, green LED emission, photodiode and filters, excitation LED 525 nm,pre-calibrated intensity • Smart Fluo-Sensors, blue LED emission, photodiode and filters, excitation LED 465 nm, • pre-calibrated intensity • Filter Sets (Mg green/Ca Green, Amplex Red, Safranin) consisting of LED filters and photodiode filters TIP2k-Module: add-on for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control • Microinjection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tips • Needle Spacers, silicone stops (200/Pkg.) with mounting tool • TIP2k-Needle Safety Support with cable guide • TIP2k-Filter Papers O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function • Duran glass chambers, with smaller operation volume (V) of 0.5 cm3 (0.5 mL) • PVDF stirrers for small volume chambers, electromagnetic control • PEEK stoppers for small volume chambers • Black POM chamber holders with O-ring and V-ring • Black POM OroboPOS-holders Item 2: Power Package needed to increase throughput. Integrated computer with touchscreen connects DatLab to the O2k Keyboard, webcam, and webcam accessories O2k: Hardware and DatLab Softwar O2k: Accessories and Consumables • MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL O2k-Fluo Smart-Module: an amperometric add-on module for measurement of hydrogen peroxide production, ATP production, mt-membrane potential, Ca2+ TIP2k-Module: add-on for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

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