ORGAN BATH SYSTEM

Procurement Summary

The National Institute on Aging (NIA) requires an organ bath system to measure various aspects of vasculature studied from mouse models of the chronological onset of senescence in the aging vasculature, and atherosclerosis -induced cellular senescence on vascular cognitive impairment and dementia. The organ bath system is a more complex device that allows researchers to study the intact blood vessel and its surrounding tissues in a physiological environment. The system consists of a chamber filled with a physiological solution, where a blood vessel is mounted and subjected to different experimental stimuli, such as neurotransmitters or drugs. The system provides measurements of vascular diameter and tension, enabling researchers to study the complex interactions between different cell types and surrounding tissues in vascular function. The organ bath system is particularly useful for studying larger blood vessels and investigating the effects of different experimental conditions on vascular responses. The salient characteristics of the required organ bath system are as follows: - Chamber volume range of 4.0 ml – 8.0 ml - 4 separate chambers - Chamber material must be acid resistant stainless steel - Tissue size for ring mount a minimum of 250 µm - Tissue size for strip mount a minimum of 1 mm - Mounting type for tissue either pins or clamps - Temperature range for the bath must be between 15.0 – 50.0 oC with a resolution of 0.1 oC and a stability of +/- 0.2 oC. - The transducer must have an output reading in either mN or g with a range of +/-200/+/- 400/+/-800/+/-1600 mN with a resolution of 0.01 mN. - Force output data should be available in both analog or digital format - The dimensions (LWD) of the instrument must be able to fit within 50 x 30 x 20 cm range - Software must be included - The force transducers should be programmable - Built-in vacuum - Option for gas bubbling - Automatic refilling of chambers - Option for addition of compounds to chambers during testing

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