AUTOMATED FLOW CYTOMETER

Procurement Summary

The mission requires one (1) automated flow cytometer which is intended to satisfy a requirement. The automated features for the novel benchtop cell analyzer are for highly sensitive multicolor flow analysis. Anticipated mission impact if there is no waiver; The automated flow cytometer is crucial to the mission of to the mission of the lab as it enables reliable tissue dissociation or thorough homogenization to use in virtually any cell research fi eld, including cancer, immunology, neuroscience, stem cell, and cardiovascular research The labs research would be severely impacted regarding the time it would take to run the experiments without the purchase of the automated flow cytometer. This item is required to continue processing these samples in IMVSBSL2 laboratory. IMVS needs this instrument based on the need for continuity across experiments. Automated Flow Cytometer Specifications: Optics Laser excitation Spatially separated: 405 nm,65 mW 488 nm, 50 mW 640 nm, 72 mW Emission detectors FSC:488/10 nm SSC: 405/10 nm B1: 525/50 nm B2: 579/34 nm B3:615/20 nm B4: 667/30 nm B5: 725/40 nm B6: 785/62 nm V1: 450/50nm V2: 525/50 nm V3: 579/34 nm V4: 615/20 nm V5: 667/30 nm R1:667/30 nm R2: 725/40 nm R3: 785/62 nm Flow cell dimensions200×250 μm Fluorescence detectors Optimized with spectrally matched PMTs for all channels Fluidics Minimal uptake volume 1 μL(25 μL recommended for volumetric counting applications) Sample flow rate 25, 50, or 100 μL/min or automate flow rate to maintain 500,1000, or 2000 events/second Measurement speed <25 minutes per96-well plate (5 μL measurement volume per well) Sample uptake 1–450 μL Maximal event rate Up to 15,000 events/second Technical Specifications Footprint 385.5 × 284.5 mm (15.18 × 11.2 in) Size(width×depth) Height (adjustable touchscreen) 669 × 400 mm (26.34× 15.75 in) 394–553.5 mm (15.51–21.79 in) Size with mini Sampler (width × depth) 669×500 mm (26.34 × 19.69 in) Weight 50 kg (110lbs.) Monitor 15.6" LCD touchscreen Power requirements 100–240V~, 50/60 Hz Power consumption (standard use) 450 W RAM 8 GBDDR4 (SO-DIMM) Mass storage 500 GB SSD Ports 4× USB 2.0 ports,6× USB 3.0 ports (2 at display), 2× DisplayPort, 2× LAN, DVI, RE-232,Audio Emission sound pressure level at workstation <61 dB(A)Performance Sample carry-over 0.01% Fluorescence performance 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales Data Management Operating system Embedded operating system Measurement parameters Area, width, height for all parameters, with time and volume Signal processing >18 bit dynamic range in area with 32 bit floating point signal processing Compensation Automated, or manual with 14×14 matrix, during or after acquisition Data fi les .mqd (proprietary fi le format), .fcs (2.0,3.0, 3.1 compatible).

Yes

Yes