Long-read sequencing Promethion flow cells
Waiver type - Procurement: Nonavailability
Agency: NATIONAL INSTITUTES OF HEALTH (7529) • Product Service Code (PSC) : 6505 | Last Modified: 08/12/2024
Procurement Summary
The purpose of this request is to purchase long-read sequencing Promethion flow cells. These flow cells are consumables which are required to use the Nanopore PromethION sequencing instrument which generates the long-read sequencing data. Nanopore flow cells ordered in this purchase will be used for several collaborative projects between CBGC and other IRP researchers in NIA. The projects include studying age-associated changes in gene expression and epigenetics (DNA methylation) in cell lines and in various animal tissues. RNA and DNA isolated from these samples will be sequenced using full-length RNA/cDNA-seq to identify transcript isoforms, splice variants and using long-read DNA-seq to map global DNA methylation.
Waiver Rationale Summary
The mission of the Computational Biology and Genomics Core (CBGC) of NIA is to support collaborative projects with researchers requiring long-read sequencing of RNA, cDNA and DNA for in-depth transcriptomic analysis. CBGC achieves this Core function through a Promethion Sequencing instrument purchased for the Core for this purpose. The Promethion Sequencer requires these Promethion flow cells to be used with Nanopore sequencing technology, which has been in use for several years now and has become optimized for use in in-house sequencing.
Yes
Yes
Consistent with Policy
DRUGS AND BIOLOGICALS
325414 - Biological Product (except Diagnostic) Manufacturing
08/02/2024
N/A
N/A
Pre-solicitation
0 - 6 months
Individual Waiver
NATIONAL INSTITUTES OF HEALTH