Stunner Platform – benchtop micro-volume and high throughput protein characterization system

Procurement Summary

NIAID requires 1x Qty of Part#700-2002; Description: Stunner Platform. This item is manufactured by Labs in Belgium. National Institute of Allergy & Infectious Diseases (NIAID)’s Vaccine Research Center (VRC) (collectively “Program”) is engaged in research to facilitate the development of vaccines against HIV/AIDS and other infectious pathogens/diseases. Program’s goal is to develop immunogens that will elicit broadly neutralizing antibodies and will be used in vaccine design. This research requires equipment that are capable of protein characterization in the form of micro-volume and high throughput protein characterization systems. Program has evaluated one such system called the Stunner Platform created by Unchained Labs, and determined that this system suits its needs for research. This acquisition has certain technical requirements. These requirements include the following: Crucial must have features: -UV/Vis -Dynamic light scattering (DLS) -Static light scattering (SLS) -Minimal amount of sample (< 5 uL) Good to have but not absolutely necessary features: -LNP total RNA quant -AAV & AdV capsid titer Max dimensions ‐ 37 cm W x 54 cm D x 33 cm H; <35 kg UV/Vis specifications Light source ‐ Xenon flash lamp Detectors ‐ UV/Vis polychromatic spectrophotometer Wavelength ‐ range230‐750 nm Wavelength accuracy ‐ ≤400nm: ±1nm; ≥400nm: ±2 nm Spectral resolution ‐ Better than 2 nm (toluene in hexane) Absorbance precision (1 cm quartz cuvette) <1 OD: ±0.005 OD; st dev 1‐2 OD: ±0.5% CV Absorbance accuracy (1 cm quartz cuvette) <1 OD: ±0.01 OD; 1‐2 OD: ±1% DLS specifications Light source ‐ 660 nm laser diode Detection ‐ Avalanche photodiode module Size accuracy ‐ ±2% Minimum sample concentration ‐ 0.1 mg/mL lysozyme Hydrodynamic diameter range ‐ 0.3–1000nm Samples per plate 96 (12 x 8 microplate format) Measurement time for full plate ‐ <20 minutes for UV/Vis only; <90 min for UV/Vis and DLS Measurement range: OD 10 mm ‐ 0.03–275 OD ng/μL dsDNA ‐ 1.5–13750 ng/μL mg/mL ave protein mix ‐ 10 mm 0.03–275 mg/mL Absorbance precision (10 mm pathlength) <1 OD: ±0.01 OD; st dev 1‐200 OD: ±1% CV Absorbance accuracy (10 mm pathlength)<1 OD: ±0.02 OD; 1‐200 OD: ±2% To our knowledge, only this Stunner platform from Unchained Labs can meet the requirements of Program, and this platform is made in a foreign country of Belgium.

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